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The full-length influenza haemagglutinin or the head domain alone of <t>B/Phuket/3073/2013</t> <t>or</t> <t>B/Washington/02/2019</t> was used as the immobilised antigen in the ELISA pulldown, and eluted antibodies were tested for neutralisation of the corresponding or a mismatched control pseudotype virus. (A) Proportion of sera with resolvable ID 50 values by antigen and virus. (B) Median ID 50 values of sera with resolvable neutralisation. ID 50 values for antibodies purified against full-length HA are shown in blue; those purified against the head domain alone are shown in red. Statistical analysis was performed using Kruskal-Wallis tests with Dunn’s correction.
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The full-length influenza haemagglutinin or the head domain alone of B/Phuket/3073/2013 or B/Washington/02/2019 was used as the immobilised antigen in the ELISA pulldown, and eluted antibodies were tested for neutralisation of the corresponding or a mismatched control pseudotype virus. (A) Proportion of sera with resolvable ID 50 values by antigen and virus. (B) Median ID 50 values of sera with resolvable neutralisation. ID 50 values for antibodies purified against full-length HA are shown in blue; those purified against the head domain alone are shown in red. Statistical analysis was performed using Kruskal-Wallis tests with Dunn’s correction.

Journal: bioRxiv

Article Title: Development of an ELISA-Based Pulldown Approach for Functional Analysis of Antigen-Specific Antibodies

doi: 10.64898/2026.02.19.706726

Figure Lengend Snippet: The full-length influenza haemagglutinin or the head domain alone of B/Phuket/3073/2013 or B/Washington/02/2019 was used as the immobilised antigen in the ELISA pulldown, and eluted antibodies were tested for neutralisation of the corresponding or a mismatched control pseudotype virus. (A) Proportion of sera with resolvable ID 50 values by antigen and virus. (B) Median ID 50 values of sera with resolvable neutralisation. ID 50 values for antibodies purified against full-length HA are shown in blue; those purified against the head domain alone are shown in red. Statistical analysis was performed using Kruskal-Wallis tests with Dunn’s correction.

Article Snippet: B/Phuket/3073/2013 and B/Washington/02/2019 full length Influenza HA were obtained from Sino Biological.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Virus, Purification